Seth Green
The Nerd / Batman / Robin / Aquaman / The Penguin / Martian Manhunter / Toyman / Scarecrow / Gorilla Grodd / Little Cheese / Swamp Thing / Abin Sur / Ganthet / Green Arrow / Hawkman / Jimmy Olsen / Various (voice)
Breckin Meyer
Superman / Mirror Master (voice)
Nathan Fillion
Green Lantern / Mr. Freeze (voice)
Alex Borstein
Wonder Woman / Giganta / Woman (voice)
Matthew Senreich
The Flash / Chillblaine / Brainiac / Wildcat (voice)
Alfred Molina
Lex Luthor / Firestorm / Mr. Banjo (voice)
Paul Reubens
The Riddler (voice)
Neil Patrick Harris
Two-Face / Black Manta (voice)
Kevin Shinick
Narrator / Captain Cold (voice)
Abraham Benrubi
Cyborg/ Kilowog / Appa Ali Apsa / Solomon Grundy (voice)
Zeb Wells
Sinestro / Swamp Thing (voice)
Megan Fox
Lois Lane (voice)
Tara Strong
Harley Quinn / Selena Gomez (voice)
Tom Root
Icicle/B'dg (voice)