Spencer Tracy
Dr. Henry 'Harry' Jekyll / Mr. Hyde
Ingrid Bergman
Ivy Peterson
Lana Turner
Beatrix 'Bea' Emery
Donald Crisp
Sir Charles Emery
Ian Hunter
Dr. John Lanyon
Barton MacLane
Sam Higgins
C. Aubrey Smith
The Bishop
Frederick Worlock
Dr. Heath
William Tannen
Intern Fenwick
Forrester Harvey
Old Prouty
Lumsden Hare
Colonel Weymouth
Lawrence Grant
Dr. Courtland
Hillary Brooke
Mrs. Arnold (uncredited)
Alec Craig
Tripped Waiter (uncredited)
Brandon Hurst
Dr. Lanyon's Butler Briggs (uncredited)
Olaf Hytten
Hobson (uncredited)
Colin Kenny
Constable (uncredited)
Doris Lloyd
Mrs. Marley (uncredited)
Aubrey Mather
Inspector (uncredited)
Al Ferguson
Constable (uncredited)
Mary Field
Wife (uncredited)
Frank Hagney
Drunk (uncredited)
Bobby Hale
Cart Driver (uncredited)
Martha Wentworth
Landlady (uncredited)