Ben Diskin
Nigel "Numbuh One" Uno / Hoagie Pennywhistle "Numbuh Two" Gilligan Jr. / D.C.F.D.T.L. (Voice)
Lauren Tom
Kuki "Numbuh Three" Sanban (Voice)
Dee Bradley Baker
Wallaby "Numbuh Four" Beatles / D.C.F.D.T.L. / Chip / Toiletnator / Mr. Fibb (Voice)
Cree Summer
Abigail "Numbuh Five" Lincoln / D.C.F.D.T.L. (Voice)
Maurice LaMarche
Father (Voice)
Ric Stoneback
Adult Hoagie
Jeff Bennett
Mr. Boss (Voice)
Jennifer Hale
Computer / Mrs. Uno / Numbuh 86 / Numbuh 363 (Voice)
Mark Hamill
Stickybeard (Voice)
Janice Kawaye
Sonia / Lee (Voice)
Tom Kenny
Mr. Dink / Knightbrace (Voice)
Rachael MacFarlane
Numbuh 362 (Voice)
Frank Welker
Mr. Uno (uncredited) (Voice)
Candi Milo
Lydia Gilligan (Voice)
James Arnold Taylor
Nick (Voice)
Daran Norris
Count Spankulot (Voice)
Rob Paulsen
Robin Food (Voice)
Fred Tatasciore
Cheese Shogun Roquefort (Voice)
Dave Wittenberg
Numbuh 74.239 (Voice)
