Johnny Knoxville
Luke Duke
Seann William Scott
Bo Duke
Jessica Simpson
Daisy Duke
Willie Nelson
Uncle Jesse Duke
Burt Reynolds
Jefferson Davis 'Boss' Hogg
M.C. Gainey
Sheriff Rosco P. Coltrane
James Roday Rodriguez
Billy Prickett
David Koechner
Cooter Davenport
Michael Weston
Deputy Enos Strate
Nikki Griffin
Katie Johnson
Alice Greczyn
Laurie Pullman
Joe Don Baker
Governor Jim Applewhite
Jack Polick
Deputy Cletus Hogg
Therial 'Houseman' DeClouet
Judge Locke Randolph
Jim Cody Williams
Security Guard Chip
Wayne Douglas Morgan
Prisoner
Junior Brown
The Balladeer (voice)
Christina Dare
Sorority Girl
Jay Chandrasekhar
Campus Cop #1
Erik Stolhanske
Campus Cop #2
A.J. Foyt IV
Race Car Driver #1
Mitch Braswell
Out of Towner #1