Dakota Fanning
Fern Arable
Julia Roberts
Charlotte the Spider (voice)
Steve Buscemi
Templeton the Rat (voice)
John Cleese
Samuel the Sheep (voice)
Oprah Winfrey
Gussy the Goose (voice)
Cedric the Entertainer
Golly the Goose (voice)
Reba McEntire
Betsy the Cow (voice)
Kathy Bates
Bitsy the Cow (voice)
Robert Redford
Ike the Horse (voice)
Thomas Haden Church
Brooks the Crow (voice)
André 3000
Elwyn the Crow (voice)
Dominic Scott Kay
Wilbur (voice)
Sam Shepard
Narrator (voice)
Abraham Benrubi
Uncle the Pig (voice)
Siobhan Fallon Hogan
Mrs. Zuckerman
Julian O'Donnell
Henry Fussy
Gary Basaraba
Homer Zuckerman
Teague Rook
Well Dressed Man
Robert Plazek
Ball Thrower
Joel McCrary
Sheep Group (voice)
Brian Stepanek
Sheep Group (voice)
Fred Tatasciore
Sheep Group (voice)
Bradley White
Sheep Group (voice)
Maia Kirkpatrick
Joy the Baby Spider (voice)
Jennessa Rose
Aranea the Baby Spider (voice)
Briana Hodge
Nellie the Baby Spider (voice)
Dale Azzopardi
Photographer (uncredited)
Ella Scott Lynch
Girl at Fair (uncredited)